Methods and materials for producing gene libraries
US5869295A · kind A · utility
2Cited by
5References
13Claims
0Family size
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Key dates
| Filing date | Dec 20, 1996 |
| Grant date | Feb 9, 1999 |
| Priority date | — |
| Expiry date | Dec 20, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/66
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for producing libraries of diverse nucleotide sequences, and libraries of polypeptides encoded thereby, are provided. The nucleotide sequences comprise a first and a second constant region coupled to a coding sequence, wherein the coding sequence is formed by sequentially coupling nucleotides in a mixture of predetermined proportions of A, T, C, and G based upon a known amino acid profile. Libraries of vectors comprising the diverse nucleotide sequences are also provided. DNA and amino acid sequences encoding the libraries are further provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.