Crystallization and purification of polypeptides
US5869604A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 9, 1995 |
| Grant date | Feb 9, 1999 |
| Priority date | — |
| Expiry date | Nov 9, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K1/36
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention is directed to a method of highly purifying a polypeptide or other biological compounds from a contaminant by crystallization comprising contacting a mixture containing uncrystallized polypeptides and a contaminant with an exogenous nucleating agent, crystallizing the polypeptides, thereby forming at least one crystal of the polypeptide attached to the nucleating agent, the attached crystal being of a high purity, and at least one polypeptide crystal unattached to the nucleating agent, the unattached crystal being of a lower purity than the attached crystal, and separating the crystal attached to the nucleating agent from the crystal unattached to the nucleating agent. Also, the invention is directed to a method of purifying a polypeptide in a high purity and in a high yield from a contaminant by crystallization comprising contacting a mixture containing uncrystallized polypeptides and a contaminant with an exogenous nucleating agent that has a close lattice match to the polypeptide, crystallizing the polypeptides, thereby forming at least one crystal of the polypeptide attached to the nucleating agent, the attached crystal being of a high purity and produced in a hig…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.