Synthesis of diverse and useful collections of oligonucleotidies

US5869644A · kind A · utility

Assignee

• THE JOHNS HOPKINS UNIVERSITY · US

Inventors

• David R. Shortle · Baltimore, US
• John Sondek · Chapel Hill, US

Key dates

• Filing date: Aug 8, 1996
• Grant date: Feb 9, 1999
• Expiry date: Aug 8, 2016

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/102
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A new technique for generating mixtures of oligonucleotides in a single automated synthesis is taught. The method can be used to prepare mixed oligonucleotides ideally suited for creation of useful mixtures of oligo- or polypeptides or proteins. Additionally, the technique enables insertion and/or substitution and/or deletion of a nucleotide sequence at one or more sites. For protein mutagenesis, a trinucleotide can be inserted or substituted at codon boundaries. The invented technique makes possible the encoding of all possible single amino acid insertions, or any desired mixture of substitutions and insertions.