Renibacterium salmoninarum vaccine and method for its preparation

US5871751A · kind A · utility

Assignee

• State of Oregon acting by and through the State Board of Higher Ed. on Behalf of Oregon State Univ. · US

Inventors

• John Mark Christensen · Corvallis, US
• Steve Kaattari · Yorktown, US
• Jon D. Piganelli · Pittsburgh, US
• Gregory Wiens · Portland, US
• Jia-Ai Zhang · Amherst, US

Key dates

• Filing date: Oct 12, 1994
• Grant date: Feb 16, 1999
• Expiry date: Oct 12, 2014

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S424/827
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A vaccine and method for treating fish susceptible to infection by Renibacterium salmoninarum is described. The vaccine comprises killed microorganisms that lack intact cell-surface-associated protein p57. The vaccine can be used in combination with additional materials, such as, without limitation, adjuvants, plasticizers, pharmaceutical excipients, antigens other than the cells lacking intact cell-surface-associated protein p57, diluents, carriers, binders, lubricants, glidants, aesthetic compounds, such as flavoring and coloring agents, and combinations thereof. The vaccine may be enteric-coated for oral delivery. The enteric coating generally comprises a polymer coating that is impervious to dissolution and/or degradation in the stomach, but is dissolved upon passing to the higher pH environments of the intestine. A preferred embodiment of the vaccine is made using spherical sugar microspheres. The microsphere is coated with a first layer comprising the killed Renibacterium salmoninarum microorganisms lacking intact cell-surface-associated protein p57. The sugar microsphere is then coated with a second enteric-coating layer comprising a material that is impervious to dissolutio…