Regulated transcription of targeted genes and other biological events

US5871753A · kind A · utility

Assignees

• Board of Trustees of the Leland Stanford Junior University · US
• President and Fellows of Harvard College · US

Inventors

• Gerald R. Crabtree · Woodside, US
• Stuart L. Schreiber · Cambridge, US
• David Spencer · Houston, US
• Thomas J. Wandless · Cambridge, US
• Peter Belshaw · Madison, US
• Steffan N. Ho · San Diego, US

Key dates

• Filing date: Jun 7, 1995
• Grant date: Feb 16, 1999
• Expiry date: Jun 7, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/90
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Methods and compositions are provided for modified cells, where a chimeric protein consisting of a ligand binding domain fused to an action domain is employed which initiates a signal which activates a biological process: transcription of at least one gene, usually a second construct introduced into the host cells; exocytosis; or an extracellular process. The second construct optimally present provides for a promoter which responds to a transcriptional activation action domain to provide for transcription, when an appropriate ligand binds to the ligand binding domain. Exemplary of the system is the use of an FKBP/CD3.zeta. or transcription factor fusion protein, using dimeric FK506 or FK520 as the ligand and a promoter responsive to NF-AT or other transcription factor requiring two molecules for transcriptional activation.