Process for the determination of in vitro amplified nucleic acids

US5871908A · kind A · utility

Assignee

• EVOTEC BIOSYSTEMS GMBH · DE

Inventors

• Karsten Henco · Erkrath, DE
• Manfred Eigen · Göttingen, DE
• Detlev Riesner · Düsseldorf, DE

Key dates

• Filing date: Dec 8, 1993
• Grant date: Feb 16, 1999
• Expiry date: Dec 8, 2013

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/686
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A process for the qualitative and quantitative determination of at least one in vitro amplified nucleic acid in a sealed reaction chamber, PA1 wherein during or subsequent to the amplification of the nucleic acid at least one substance (probe) is present which interacts with the nucleic acid to be detected; PA1 wherein spectroscopically measurable parameters of said substance (probe) are subject to variation, creating a measurable signal; PA1 wherein the sample to be measured is exposed to the action of a gradient capable of at least partially denaturing nucleic acids; PA1 with detection of the measurable parameter undergoing variation through the action of the gradient; and PA1 the entire amplification reaction, including qualitative and quantitative determination, may be carried out in a sealed reaction chamber (measuring compartment) without intermittent opening, permitting to automatically operate the diagnostic method of DNA and RNA amplification in qualitative and quantitative fashion on large series of samples.