Dioxetane compounds for the chemiluminescent detection of proteases, methods of use and kits therefore
US5871938A · kind A · utility
3Cited by
11References
15Claims
0Family size
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Key dates
| Filing date | Sep 22, 1997 |
| Grant date | Feb 16, 1999 |
| Priority date | — |
| Expiry date | Sep 22, 2017 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/807
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
1,2-dioxetane compounds bearing a proteolytic enzyme specific amino acid or peptide are provided, which amino acid or peptide can be removed by action of the corresponding protease. When the amino acid or peptide is removed, the 1,2-dioxetane decomposes with chemiluminescence, the generation of light providing a rapid, ultra-sensitive and convenient means for detecting the presence of the protease in the sample being inspected. The amount of light generated, or degree of chemiluminescence, can be correlated with the amount of protease present. Immunoassays, as well as DNA hybridization and DNA probe assays are provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.