Method for mass production of taxol by semi-continuous culture with Taxus chinensis cell culture
US5871979A · kind A · utility
6Cited by
5References
4Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 28, 1996 |
| Grant date | Feb 16, 1999 |
| Priority date | — |
| Expiry date | May 28, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P17/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method for mass production of taxol by semi-continuous culture of Taxus genus plant with a high yield. According to the present invention, taxol can be prepared with a high yield, by employing semi-continuous culture of Taxus genus plant cell, which comprises: (i) inoculating Taxus genus plant cell on a medium containing 1 to 10% (w/v) sugar, and incubating it; and, (ii) transferring 1/10 to 1/2 volume of the culture obtained in the step (i) to a fresh medium and repeating the step (i), adding 1 to 10% (w/v) sugar to the remnant culture and incubating to the time of maximum production of taxol.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.