DNA sequencing by mass spectrometry via exonuclease degradation
US5872003A · kind A · utility
163Cited by
3References
21Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | May 30, 1995 |
| Grant date | Feb 16, 1999 |
| Priority date | — |
| Expiry date | May 30, 2015 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N35/1067
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods for determining the sequence of nucleic acids by cleaving the nucleic acid unilaterally from a first end with an exonuclease activity to sequentially release individual nucleotides, identifying each of the sequentially release nucleotides by mass spectrometry, and determining the sequence of the nucleic acid from the identified nucleotides are disclosed. The method is amenable to multiplexing for simulataneously determining more than one nuleic acid sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.