N-terminal-marked peptides immobilized on glass beads and method of preparation and method of use thereof

US5872207A · kind A · utility

Assignee

• Sanofi Pharmaceuticals, Inc. · US

Inventors

• Barry Morgan · Franklin, US
• Mark A. Ator · Paoli, US
• James Arthur Gainor · Collegeville, US
• Thomas D. Gordon · Medway, US
• Lawrence I. Kruse · Haddonfield, US
• Teruna J. Siahaan · Lawrence, US

Key dates

• Filing date: Aug 29, 1995
• Grant date: Feb 16, 1999
• Expiry date: Aug 29, 2015

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/811
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Peptides or peptides having an amino acid-like moiety in place of an amino acid moiety thereof immobilized on glass beads, especially controlled-pore glass beads, by a C-terminal .alpha.-carboxyl-.omega.-amino linker and .alpha.-silyl-.omega.-amino anchor, especially wherein the anchor has the structural formula EQU --NH(CH.sub.2).sub.n Si(OR).sub.2 O-- wherein n is 3 and R is hydrogen or the glass bead and the linker has the structural formula EQU --NH(CH.sub.2).sub.r CO!.sub.s -- wherein r is 5 and s is an integer from 1 through 10 and marked by an N-terminal fluorescent marker, especially wherein the N-terminal fluorescent marker is 3-(7-hydroxy-4-coumarinyl)propionyl, and method of preparation thereof and method of use thereof in carrying out biochemical assays, especially wherein the immobilized peptide or peptide having an amino acid-like moiety in place of an amino acid moiety thereof is cleaved by a protease and the resulting fluorescence in the supernatant solution is measured to determine the extent of cleavage, are disclosed.