Method for electrophoretic separation

US5873991A · kind A · utility

Assignee

• Pharmacia Biotech AB · SE

Inventors

• Sven Göthe · Bromma, SE
• Anders Hult · Täby, SE
• Jan-Olof Johansson · Uppsala, SE
• Lennart Kagedal · Uppsala, SE
• Anna Sylwan · Uppsala, SE

Key dates

• Filing date: Apr 2, 1997
• Grant date: Feb 23, 1999
• Expiry date: Apr 2, 2017

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C08F220/56
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Method for electrophoretic separation of nucleic acid fragments comprising the following steps: PA1 a) providing a polyacrylamide based gel prepared by photoinitiated polymerization of a mixture containing monoolefinic- and di- and/or polyolefinic monomers and a photoinitiator of the general formula: ##STR1## where R.sub.1 and R.sub.2 independently are CH.sub.3, C.sub.2 H.sub.5 or C.sub.3 H.sub.7 or R.sub.1, R.sub.2 together with the carbon atom form a cycloaliphatic ring with 4-8 carbons; PA1 R.sub.3 =H, CH.sub.3, C.sub.2 H.sub.5 or --O--(CH.sub.2 CH.sub.2).sub.n --OH with n=1-20; PA1 b) applying said nucleic acid fragments on said polyacrylamide based gel; PA1 c) electrophoretically separating said fragments on said gel. The invention also relates to a kit for use in the method for electrophoretic separation of nucleic acid fragments.