Oligonucleotide which can be used as primer in a method of amplification based on a replication accompanied by strand displacement
US5874260A · kind A · utility
86Cited by
6References
19Claims
0Family size
Assignee
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Key dates
| Filing date | Oct 27, 1995 |
| Grant date | Feb 23, 1999 |
| Priority date | — |
| Expiry date | Oct 27, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6853
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Oligonucleotide comprising, from 5' to 3', a first portion comprising an upstream segment and a downstream segment forming a stem-loop structure, and a second portion not capable of self-pairing, in which the first portion comprises at least one blocking agent, capable of blocking the replication, by a polymerase, of the said oligonucleotide, such that at least one portion of the downstream segment is replicated and such that the upstream segment is replicated neither totally nor partially. This oligonucleotide can be used as primer in a process for the cyclic amplification of a target sequence of a nucleic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.