Method for the purification of glycosyltransferases
US5874261A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Dec 20, 1991 |
| Grant date | Feb 23, 1999 |
| Priority date | — |
| Expiry date | Dec 20, 2011 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/815
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A reiterative method for obtaining the glycosyltransferases useful for glycosyltransferase-catalyzed synthesis of a saccharide composition by serially bonding preselected saccharide units onto an immobilized acceptor moiety which is one member selected from the group consisting of proteins, glycoproteins, lipids, glycolipids and carbohydrates. The method comprises in a first iteration, the steps of (i) contacting an acceptor moiety immobilized onto a solid or semi-solid support with a mixture containing at least one glycosyltransferase under conditions effective to allow said glycosyltransferase to bind to the immobilized acceptor moiety thereby resulting in an immobilized acceptor moiety-glycosyltransferase complex, followed by (ii) contacting the immobilized acceptor moiety-glycosyltransferase complex with a preselected saccharide under conditions and in the presence of co-reagents sufficient to effect bonding of the preselected saccharide unit onto the immobilized acceptor moiety catalyzed by the glycosyltransferase thereby freeing the glycosyltransferase from the complex and obtaining an immobilized product. This is followed by a single or a plurality of subsequent iterations o…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.