Patent · US Expired

Expression of surface layer proteins

US5874267A · kind A · utility

1Cited by

1References

1Claims

0Family size

Assignee

SOLVAY (Société Anonyme) · BE

Inventors

Rolf Y. Deblaere · Waarschoot, BE
Jan Desomer · Drongen, BE
Patrick Dhaese · Zwijnaarde, BE

Key dates

Filing date	Sep 17, 1996
Grant date	Feb 23, 1999
Priority date	—
Expiry date	Sep 17, 2016

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K2319/00
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

A host cell which is provided with a S-layer comprising a fusion polypeptide consisting essentially of: PA0 (a) at least sufficient of a S-layer protein for a S-layer composed thereof to assemble, and PA0 (b) a heterologous polypeptide which is fused to either the carboxy terminus of (a) or the amino terminus of (a) and which is thereby presented on the outer surface of the said cell; can be used as a vaccine, for screening for proteins and antigens and as a support for immobilizing an enzyme, peptide or antigen. A process of transforming B. Sphaericus cells comprising electroporation is also provided.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.