Humanized green fluorescent protein genes and methods
US5874304A · kind A · utility
251Cited by
3References
81Claims
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Key dates
| Filing date | Jan 18, 1996 |
| Grant date | Feb 23, 1999 |
| Priority date | — |
| Expiry date | Jan 18, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2799/027
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed are synthetic and "humanized" versions of green fluorescent protein (GFP) genes adapted for high level expression in mammalian cells, especially those of human origin. Base substitutions are made in various codons in order to change the codon usage to one more appropriate for expression in mammalian cells. Recombinant vectors carrying such humanized genes are also disclosed. In addition, various methods for using the efficient expression of humanized GFP in mammalian cells and in animals are described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.