Culturing human pancreatic endocrine cells in medium containing extracellular matrix from human bladder carcinoma cells

US5874306A · kind A · utility

Assignee

• The Regents of the University of California · US

Inventors

• Gillian M. Beattie · Poway, US
• Alberto Hayek · Village of La Jolla, US

Key dates

• Filing date: Dec 12, 1996
• Grant date: Feb 23, 1999
• Expiry date: Dec 12, 2016

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2502/30
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Human pancreatic endocrine cells are proliferated without loss of hormone function in a culture medium containing extracellular matrix from bladder carcinoma cell lines in the substantial absence of hepatocyte growth factor/scatter factor. Proliferation is preferably carried out in the substantial absence of any peptide growth factors and nicotinamide. The cells may be proliferated in a monolayer on a solid substrate. Islets and islet-like cell clusters are proliferated without loss of insulin-secreting function by incubation in a medium containing extracellular matrix from a human bladder carcinoma cell line, preferably cell line ATCC HTB-9.