High-throughput assay
US5876946A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 3, 1997 |
| Grant date | Mar 2, 1999 |
| Priority date | — |
| Expiry date | Jun 3, 2017 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A homogeneous high throughput assay is described which screens compounds for enzyme inhibition, or receptor or other target binding. Inhibition (or binding) by the library compounds causes a change in the amount of an optically detectable label that is bound to suspendable cells or solid supports. The amounts of label bound to individual cells or solid supports are microscopically determined, and compared with the amount of label that is not bound to individual cells or solid supports. The degree of inhibition or binding is determined using this data. Confocal microscopy, and subsequent data analysis, allow the assay to be carried out without any separation step, and provide for high throughput screening of very small assay volumes using very small amounts of test compound.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.