Selection marker gene free recombinant strains: method for obtaining them and the use of these strains
US5876988A · kind A · utility
23Cited by
2References
31Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 23, 1997 |
| Grant date | Mar 2, 1999 |
| Priority date | — |
| Expiry date | Jun 23, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/65
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention discloses a selection marker free system which can be used to introduce genetic modifications in bacteria, yeasts and fungi. The system can be employed to introduce or delete desired genes or DNA fragments in the genome of the indicated host species without leaving any undesired DNA i.e. the selection marker used for selection of transformants or other DNA used for cloning. In this way strains have been developed containing only desired genes introduced at desired chromosomal sites. Similarly, desired DNA fragments have been deleted or replaced at desired sites.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.