Method for isolation of highly pure von willebrand factor

US5880265A · kind A · utility

Assignee

• Immuno Aktiengesellschaft · AT

Inventors

• Bernhard Fischer · Wien, AT
• Artur Mitterer · Orth an der Donau, AT
• Friedrich Dorner · Wien, AT
• Hans-Peter Schwarz · Wien, AT
• Peter Turecek · Klosterneuburg, AT
• Johann Eibl · Wien, AT
• Falko-Guenter Falkner · Orth an der Donau, AT
• Uwe Schlokat · Orth an der Donau, AT
• Wolfgang Mundt · Wien, AT
• Manfred Reiter · Wien, AT
• Renate Den-Bouwmeester · Wien, AT

Key dates

• Filing date: Jul 22, 1997
• Grant date: Mar 9, 1999
• Expiry date: Jul 22, 2017

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K38/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention relates to a method for isolation of highly pure von Willebrand Factor in which recombinant von Willebrand Factor (rvWF) is chromatographically purified by anion exchange chromatography on an anion exchanger of the quaternary amino type in a buffer solution comprising buffer substances and optionally salt. The buffer solutions are preferably free of stabilizers, amino acids and other additives. According to this method, highly pure recombinant vWF can be obtained, which is free from blood plasma proteins, especially free from Factor VIII, and is physiologically active. Further, the invention relates to a pharmaceutical preparation that contains rvWF, which is comprised of multimers with a high structural integrity.