Transgenic mammals expressing human coagulation factor VIII
US5880327A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 21, 1994 |
| Grant date | Mar 9, 1999 |
| Priority date | — |
| Expiry date | Sep 21, 2014 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01K2267/01
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A non-human transgenic mammalian animal, as described above, contains an exogenous double stranded DNA sequence stably integrated into the genome of the animal, which comprises cis-acting regulatory units operably linked to a DNA sequence encoding human Factor VIII protein and a signal peptide, where the cis-acting regulatory units are active in mammary gland cells and the signal peptide is active in directing newly expressed Factor VIII into the milk of the animal. The promoter may be a milk protein promoter such as for whey acidic protein, casein, lactalbumin, or beta-lactoglobulin promoter. The transgenic mammals are preferably farm animals, for example, cows, goats, sheep, rabbits and pigs. Concurrent expression of a gene for human von Willebrand's Factor into milk may be used to stabilize newly-secreted Factor VIII.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.