Multi-illumination-source flow particle analyzer with inter-location emissions crosstalk cancelation
US5880474A · kind A · utility
117Cited by
2References
13Claims
0Family size
Assignee
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Key dates
| Filing date | Aug 29, 1997 |
| Grant date | Mar 9, 1999 |
| Priority date | — |
| Expiry date | Aug 29, 2017 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2015/1438
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A multi-laser flow-cytometry system provides for inter-location emissions crosstalk cancellation, through either pulse subtraction or gating, to avoid false triggering based on shadow (pre- and post-) pulses that occur when red-excited red emissions are detected by a photodetector arranged to detect blue-excited red emissions. This overcomes a problem of undercounting of counting beads that led to misdeterminations of cell counts when blue-excited red fluorescence detections were used as a trigger. The approach also provides for more accurate quantitative data regarding fluorochrome emissions amplitudes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.