Thermococcus barossii DNA polymerase mutants

US5882904A · kind A · utility

Assignee

• Amersham Pharmacia Biotech, Inc. · SE

Inventors

• William A. Riedl · Cedarburg, US
• Susan J. Fly · Brookfield, US
• Barbara F. Kaboord · Mequon, US
• Steven H. Nye · New York, US
• Eve Y. Ting · Brookfield, US

Key dates

• Filing date: Aug 4, 1997
• Grant date: Mar 16, 1999
• Expiry date: Aug 4, 2017

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/1252
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A Thermococcus barosii DNA polymerase with reduced 3'-5' exonuclease activity is disclosed. Additionally disclosed is a Thermococcus barosii DNA polymerase with increased ability to incorporate ribonucleotides and dideoxynucleotides.