Apparatus and method for interfacing an open capillary and detector for conducting off-column analyses
US5888363A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 3, 1996 |
| Grant date | Mar 30, 1999 |
| Priority date | — |
| Expiry date | Sep 3, 2016 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44717
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An apparatus and method for interfacing a capillary with a detector is disclosed. In particular, a capillary that is used in a capillary electrophoresis technique is interfaced with an off-column detector that is destructive of the sample or that will otherwise create adverse affects on the operations of the capillary electrophoresis. By way of example only, a nitrogen chemiluminescent detector using pyro-chemiluminescent techniques is discussed. An interface between the separation and detection systems is essential to prevent interference between the two systems. The interface apparatus and method are achieved by creating a closed connection between the two systems within which the pressure and sample flow rate can be controlled. Pressure control is achieved through the introduction and venting of gas into and from the integrated system. Interference between interfaced systems is then prevented by equalizing the pressure in the interface apparatus with the head pressure on the inlet end of the capillary. Also disclosed is a device for controlling the head pressure and sample flow within the capillary thereby enabling operation of an interfaced system at non-atmospheric pressure co…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.