Process for the complete removal of protective groups on nucleoside diphosphate and triphosphate sugars with acetylesterase

US5888777A · kind A · utility

Assignee

• Novartis AG · CH

Inventors

• Reinhold Oehrlein · Rheinfelden (Baden), DE
• Gabriele Baisch · Binzen, DE

Key dates

• Filing date: Aug 6, 1997
• Grant date: Mar 30, 1999
• Expiry date: Aug 6, 2017

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P19/28
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A process for the preparation of nucleoside diphosphate and triphosphate sugars wherein hydroxyl protective groups are removed enzymatically, with acetylesterase and a process for the preparation of these sugars, which comprises coupling a nucleotide with a sugar-1-phosphate activated with a carbonyl bisazole and then removing the hydroxyl protective groups enzymatically with acetylesterase.