Devices for maintaining and growing human stem and/or hematopoietics cells
US5888807A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 7, 1995 |
| Grant date | Mar 30, 1999 |
| Priority date | — |
| Expiry date | Jun 7, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2502/99
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Methods, compositions and devices are provided for the growth of human stem and/or hematopoietic cells in culture. Bioreactors are provided in which diverse cell types are simultaneously-cultured in the presence of appropriate levels of nutrients and growth factors substantially continuously maintained in the bioreactor while removing undesirable metabolic products. This simultaneous culture of multiple cell types successfully reconstructs hematopoietic tissue ex vivo. Optionally, at least one growth factor is provided through excretion by transfected stromal cells, particularly heterologous cells. The invention also allows for the separate maintenance of stromal and hematopoietic cells, and to allow for harvesting of both the adherent and non-adherent cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.