Nucleic acid molecules encoding non-infectious, non-replicating, HIV retrovirus-like particles containing heterologous antigenic anchor sequences
US5889176A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 6, 1996 |
| Grant date | Mar 30, 1999 |
| Priority date | — |
| Expiry date | Dec 6, 2016 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2469/20
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Non-infectious, retrovirus-like particles comprise an assembly of an env gene product, a pol gene product and a gag gene product contain an antigenic marker which is non-retroviral or non-HIV retroviral. In one embodiment, the marker comprises an amino acid sequence containing an epitope inserted into the gag gene product at an antigenically-active insertion site. In another embodiment, the marker comprises an antigenic anchor sequence operatively connected to the env gene product replacing endogenous anchoring function. The corresponding nucleic acid molecules are described. The non-infectious, retrovirus-like particles have utility in in vivo administration including to humans and in diagnosis. The presence of the antigenic marker enables recognition that antiserum containing anti-retroviral antibodies has been generated by exposure to the non-infectious retrovirus-like particles by testing for antibodies specific to the antigenic marker.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.