Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks
US5891313A · kind A · utility
25Cited by
8References
8Claims
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Key dates
| Filing date | Nov 8, 1995 |
| Grant date | Apr 6, 1999 |
| Priority date | — |
| Expiry date | Nov 8, 2015 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44747
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A capillary electrophoresis system and method of electrokinetically loading a capillary electrophoresis sample into a separation medium in a capillary tube in which an entangled polymer matrix is formed having the sample embedded therein. The matrix has a mesh size effective to retard movement of macromolecules such as DNA sequencing templates through the matrix when an electric field is applied across the matrix. The entangled polymer matrix is formed by a linear polymer having a molecular weight of at least 20K Daltons. Furthermore, the invention includes stable denaturants useful for the electrophoresis of nucleic acids.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.