Compositions for improving RNase cleavage of base pair mismatches in double-stranded nucleic acids
US5891629A · kind A · utility
3Cited by
9References
51Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Sep 28, 1995 |
| Grant date | Apr 6, 1999 |
| Priority date | — |
| Expiry date | Sep 28, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6827
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention addresses compositions and methods for cleaving nucleic acids. The invention allows one to reliably detecting point mutations in long and short target regions of nucleic acids in a safe, non-labor intensive, and cost effective manner. The methods and compositions of the present invention allow for the use of various RNases, such as RNase A, RNase I and RNase T2, in the detection of mutations. The present invention also identifies reaction conditions that result in significant improvement in specific mismatch cleavage in the NIRCA.TM. assay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.