Method for the immunoassay of antithrombin III activated by a glycosaminoglycan, corresponding monoclonal antibodies and method for obtaining them
US5891647A · kind A · utility
6Cited by
0References
22Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 11, 1996 |
| Grant date | Apr 6, 1999 |
| Priority date | — |
| Expiry date | Oct 11, 2016 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/8128
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method that assays activated antithrombin III (AT III) is useful for monitoring the effect in vivo of an agent that activates AT III. It involves the steps of contacting AT III with a solution sample containing the agent, wherein the agent is a sulfated glycosaminoglycan or a sulfated oligosaccharide, followed by contacting the sample with i) a first monoclonal antibody that preferentially binds AT III activated by the agent, as opposed to binding native AT III, and ii) a second monoclonal antibody that preferentially binds denatured AT III as opposed to either AT III activated by the agent or native AT III.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.