Modified bacterial alkaline phosphatases and their applications

US5891699A · kind A · utility

Assignee

• COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES · FR

Inventors

• Jean-Claude Boulain · Palaiseau, FR
• Laurence Cattolico · Sceaux, FR
• Frederic Ducancel · Longjumeau, FR
• Andre Menez · Voisins-le-Bretonneux, FR

Key dates

• Filing date: Jun 28, 1996
• Grant date: Apr 6, 1999
• Expiry date: Jun 28, 2016

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/849
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Modified alkaline phosphatases of bacterial origin (BAP or bacterial alkaline phosphatase) which consist of a bacterial alkaline phosphatase sequence in which at least one of the amino acid residues in position 329 or in position 330 is replaced by another amino acid residue, which modified bacterial alkaline phosphatases exhibit both significantly improved enzymic properties and an increased thermal stability, and their applications, in particular in immunoenzymic assays (reagents and diagnostic kits). Method for selecting mutants of alkaline phosphatases which possess significantly improved enzymic properties.