Isolated native primate GM-CSF protein
US5895646A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Nov 23, 1994 |
| Grant date | Apr 20, 1999 |
| Priority date | — |
| Expiry date | Nov 23, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S930/145
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method for purifying CSF protein is described. The method comprises: precipitating the protein with ammonium sulfate at 80% saturation to form a pellet containing the CSF protein; resuspending the pellet in a buffered solution at a pH in the range of about 6 to about 8; applying the buffered solution containing CSF to a chromatographic column, eluting with the buffered solution containing sodium chloride and collecting the fractions having CSF activity; pooling the active fractions, applying them to a C4 reverse phase column and eluting with a 0 to 90% acetonitrile gradient to collect the active fractions. The purified CSF protein has a specific activity of at least about 1.times.10.sup.7 units per mg of protein and preferably at least about 4.times.10.sup.7 units per mg of protein when assayed using the human bone marrow assay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.