Method for in vitro protein synthesis
US5895753A · kind A · utility
3Cited by
3References
8Claims
0Family size
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Key dates
| Filing date | Sep 30, 1997 |
| Grant date | Apr 20, 1999 |
| Priority date | — |
| Expiry date | Sep 30, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for performing coupled in vitro transcription and translation reactions is disclosed In the transcription reaction, the quantity of mRNA produced is limited to a level of less than about 2.5 micrograms in a 10 microliter volume prior to the translation elements being added. Limiting the level of mRNA produced prevents saturation of the translational processes and thus aids in the efficiency and fidelity of the translation process.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.