Method and apparatus for the removal of non-uniformities in an electrophoresis apparatus
US5897760A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 10, 1997 |
| Grant date | Apr 27, 1999 |
| Priority date | — |
| Expiry date | Mar 10, 2017 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44721
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method and apparatus for correcting non-uniformities in the source and the lens assembly of an electrophoresis apparatus is provided. Assuming a detector with a uniform responsivity, correcting for source and lens non-uniformities allows quantitative measurements of an electrophoresis gel to be made, thus increasing the information which can be obtained from an electrophoretic analysis. The non-uniformities due to the illumination source are characterized by sampling a portion of the source with a linear detector array and creating a correction data file. In order to sample the source, a mirror or beamsplitter is appropriately positioned, for example along the central optical axis of the electrophoresis apparatus. Similarly, a correction data file representing the non-uniformities due to the lens assembly is created using a secondary linear source of known uniformity. After the correction data files are stored, an image of the sample is taken and a sample data file is created. The sample data file can be normalized using the correction data files thereby creating a corrected sample file which can either be displayed or stored for future use.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.