Rapid detection of analytes with receptors immobilized on soluble submicron particles
US5898005A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 13, 1994 |
| Grant date | Apr 27, 1999 |
| Priority date | — |
| Expiry date | Apr 13, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/823
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods are provided for conducting specific binding assays to determine the concentration or presence of at least one analyte in a sample. Dendrimer-reagent preparations with particular analyte specificities are mixed in solution with a sample to form dendrimer-reagent-sample complexes. The complexes are then immobilized on a solid phase. Immobilization is facilitated by coupling specific binding assay reagents such as polypeptide receptors or analytes with water soluble polymers. Such water soluble polymers, for example star polymers such as dendrimers, provide production advantages of lot-to-lot uniformity and homogeneity, and can enhance sensitivity due to low non-specific binding to the solid phase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.