DNA purification and isolation using magnetic particles
US5898071A · kind A · utility
205Cited by
5References
13Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Jan 2, 1998 |
| Grant date | Apr 27, 1999 |
| Priority date | — |
| Expiry date | Jan 2, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of separating polynucleotides, such as DNA, RNA and PNA, from a solution containing polynucleotides by reversibly and non-specifically binding the polynucleotides to a solid surface, such as a magnetic microparticle, having a functional group-coated surface is disclosed. The salt and polyalkylene glycol concentration of the solution is adjusted to levels which result in polynucleotide binding to the magnetic microparticles. The magnetic microparticles with bound polynucleotides are separated from the solution and the polynucleotides are eluted from the magnetic microparticles.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.