Induction of embryogenesis using cytoskeleton inhibitors or protein synthesis inhibitors
US5900375A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Mar 29, 1996 |
| Grant date | May 4, 1999 |
| Priority date | — |
| Expiry date | Mar 29, 2016 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01H4/00
- WIPO fieldFood chemistry
- WIPO sectorChemistry
Abstract
Embryogenesis from plant microspores is routinely induced with a 16-24 h temperature treatment of 32.5.degree. C. Continuous culture at 25.degree. C. results in pollen development. However, microspore treatment with anti-cytoskeletal agents, or protein synthesis inhibitors, at the non-inductive temperature of 25.degree. C., can induce embryogenesis, thus demonstrating that heat shock is not required for embryogenic induction. Furthermore, when anti-microtubule agents (e.g. colchicine) are used, embryo induction and chromosome doubling occur simultaneously, thus generating doubled haploids, whereas heat induction generates haploids. Thus, the use of microtubule inhibitors will provide a simple one-step process to simultaneously induce embryogenesis and chromosome doubling for the production of fertile plants, thus providing minimal manipulation which will be very advantageous for genetic studies and plant breeding programs. As noted, heat shock induces haploids. A low level of chromosome doubling can be obtained by adding colchicine to microspore cultures during the heat treatment. However, the use of trifluralin with the heat treatment, to generate doubled haploid plants results in…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.