Cultured inner cell mass cell lines derived from bovine or porcine embryos

US5905042A · kind A · utility

Assignee

• University of Massachusetts, A Public Institution of Higher Education of the Commonwealth of Massachusetts, as Represented by its Amherst Campus · US

Inventors

• Steven Stice · Granby, US
• Paul Golueke · Granby, US

Key dates

• Filing date: Apr 1, 1996
• Grant date: May 18, 1999
• Expiry date: Apr 1, 2016

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2510/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Novel cultured inner cell mass (CICM) cells, and cell lines, derived from ungulates, in particular, pigs and cows, and methods for their preparation are provided. The subject CICMs possess similar morphology and express cell markers identically or substantially similarly to ICMs of undifferentiated developing embryos for prolonged culturing periods. Heterologous DNAs may be introduced into the subject CICM cells and cell lines to produce transgenic cells useful for the production of transgenic embryos, fetuses and/or offspring, e.g., by nuclear transfer procedures.