Culture skin and process for preparing the same
US5906937A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 5, 1996 |
| Grant date | May 25, 1999 |
| Priority date | — |
| Expiry date | Mar 5, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/54
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for preparing a culture skin which comprises the following steps: PA1 a) a step in which a culture skin matrix comprising a collagen sponge, a collagen sheet or a collagen gel is prepared in a means having a projection to provide said matrix with a penetrating pore, PA1 b) a step in which a skin-derived cell is seeded and cultured on said matrix, and PA1 c) a step in which the culture skin is provided with a penetrating pore by the means having the projection before said culture skin covers a skin defect, if necessary, the steps a) and b) are simultaneously carried out; a culture skin matrix and a culture skin obtained thereby. According to the present invention, a culture skin can be prepared wherein sufficient nutrition supply to epidermal cells and/or fibroblasts cultured and proliferated and discharge of exudate excessively pooled in the wound can be smoothly performed, while contraction is inhibited, by producing a penetrating pore in the culture skin before covering the wound with the culture skin with a means which requires no pretreatment in order to prevent the flow and loss of cells which are caused in seeding cells on a collagen matrix.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.