Cultured fibroblast or epithelial cell dissociation method using sulfated polysaccharide and chelator
US5906939A · kind A · utility
2Cited by
2References
6Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | May 2, 1997 |
| Grant date | May 25, 1999 |
| Priority date | — |
| Expiry date | May 2, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2509/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A cultured eukaryotic cell dissociation method using at least one polysaccharide polymer derivative. In particular, the method of dissociating cultured, adherent fibroblast or epithelial cells using a chelating agent such as EDTA and a sulfated polysaccharide such as heparin is disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.