Aqueous two-phase metal affinity partitioning protein purification system
US5907035A · kind A · utility
7Cited by
1References
28Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | May 22, 1997 |
| Grant date | May 25, 1999 |
| Priority date | — |
| Expiry date | May 22, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K1/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to methods of purifying proteins having surface active, electron-rich amino acids using an aqueous two phase system. The methods include the use of salts and inert hydrophobic molecules, such as polymers, to produce the aqueous two phase system and the use a polymer-chelator-metal complex to purify the target proteins.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.