Methods for measuring cellular proliferation and destruction rates in vitro and in vivo
US5910403A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | May 15, 1997 |
| Grant date | Jun 8, 1999 |
| Priority date | — |
| Expiry date | May 15, 2017 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/60
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to methods for measuring the proliferation and destruction rates of cells by measuring deoxyribonucleic acid (DNA) synthesis. In particular, the methods utilize non-radioactive stable isotope labels to endogenously label DNA synthesized through the de novo nucleotide synthesis pathway in a cell. The amount of label incorporated in the DNA is measured as an indication of cellular proliferation. Such methods do not involve radioactivity or potentially toxic metabolites, and are suitable for use both in vitro and in vivo. Therefore, the invention is useful for measuring cellular proliferation in humans for the diagnosis of a variety of disease conditions in which cellular proliferation is involved.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.