Method for sequencing nucleic acids

US5912118A · kind A · utility

Assignee

• Europaeisches Laboratorium Fuer Molekularbiologie (EMBL) · DE

Inventors

• Wilhelm Ansorge · Gaiberg, DE
• Hartmut Voss · Schriesheim, DE

Key dates

• Filing date: Apr 18, 1994
• Grant date: Jun 15, 1999
• Expiry date: Apr 18, 2014

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/143333
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

In order to sequence nucleic acids one produces a mixture of labelled nucleic acid fragments of different length, using nucleic acid fragments which are labelled by incorporation of at least one deoxyribonucleoside triphosphate with a non-radioactive labelling group, separates the labelled nucleic acid fragments according to size and determines the nucleic acid sequence by means of the labelling of the individual fragments.