Padlock probe detection
US5912124A · kind A · utility
170Cited by
4References
20Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Jun 14, 1996 |
| Grant date | Jun 15, 1999 |
| Priority date | — |
| Expiry date | Jun 14, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6834
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the detection of a polynucleotide using a padlock probe, whereby a hybridization process is combined with ligation to irreversibly capture polynucleotides of interest. The invention relates both to the padlock probe itself, as well as to methods of use, which allow for varying levels of sensitivity due to amplification of signal processes built into the system. Additionally, preferred formats are disclosed for use of the padlock probes, including the use of microfluidics.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.