Nucleic acid analogue assay procedures and kits
US5912145A · kind A · utility
53Cited by
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14Claims
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Key dates
| Filing date | Mar 1, 1996 |
| Grant date | Jun 15, 1999 |
| Priority date | — |
| Expiry date | Mar 1, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6851
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An assay procedure to quantify the amount of starting template in a nucleic acid amplification procedure such as PCR involves trying the amplification in the presence of varying amounts of a nucleic acid analogue which in sufficient quantity will inhibit said amplification procedure by selective interaction with a nucleic acid species present, e.g. a PNA as described in WO-02/20703. The amount of template is given by the amount of nucleic acid analogue critical to cause failure of said amplification procedure.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.