Method for synthesis of nucleic acids
US5912146A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 15, 1997 |
| Grant date | Jun 15, 1999 |
| Priority date | — |
| Expiry date | Aug 15, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6806
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method of nucleic acid synthesis capable of directly amplifying a gene of interest in a salivary sample without purifying DNAs from the sample. According to the present invention, in a method of nucleic acid synthesis by mixing a salivary sample itself and a gene amplification reaction solution, and then subjecting them to an amplification reaction, the salivary sample is heat-treated or treated with a saccharide-degrading enzyme before the reaction. For example, a salivary sample was heat-treated at 0-40.degree. C. for 1 hour, subsequently added directly into a PCR reaction solution, and then subjected to a PCR. The result demonstrates that, as shown in FIG. 3, the amount of the PCR product increased according as the heat-treatment temperature was elevated, and as a consequence, the PCR product sufficient to be detected in the electrophoresis was produced in each of all cases using different amounts of saliva, by heat-treating the sample at 30.degree. C. or above.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.