Patent · US Expired

Multimeric self-cleaving ribozyme

US5912149A · kind A · utility

3Cited by
1References
14Claims
0Family size

Assignee

Inventors

Key dates

Filing dateSep 25, 1996
Grant dateJun 15, 1999
Priority date—
Expiry dateSep 25, 2016

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N2310/53
  • WIPO fieldPharmaceuticals
  • WIPO sectorChemistry

Abstract

The invention pertains to a multimeric self-cleaving ribozyme comprising multiple repeating units, wherein each unit comprises a ribozyme containing a catalytic domain and an antisense domain, and a target recognition sequence or portion thereof recognized by the antisense domain of the ribozyme and comprising a ribozyme cleavage site to facilitate self-cleavage. The invention also pertains to constructs comprising DNA encoding the multimeric self-cleaving ribozyme, cells stably transformed with these constructs, and methods of cleaving RNA, regulating gene expression and generating molecular weight ladders utilizing the multimeric self-cleaving ribozyme.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.