High flow technique for harvesting mammalian cells

US5912163A · kind A · utility

Assignee

• Circe Biomedical, Inc. · US

Inventors

• Kermit M. Borland · Shrewsbury, US
• Barbara A. Chandler · Lexington, US
• Dianna Hunt Picton · Bedford, US
• Shawn P. Cain · Chelmsford, US
• Deborah Deane · Orange, US
• Claudy J. Mullon · Framingham, US

Key dates

• Filing date: Oct 20, 1997
• Grant date: Jun 15, 1999
• Expiry date: Oct 20, 2017

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12M45/09
• WIPO field: Basic materials chemistry
• WIPO sector: Chemistry

Abstract

The invention is based in part on the observation that significant portions of porcine livers appear to remain intact after perfusion by standard methods, suggesting that the perfusion procedures employed do not result in complete enzymatic digestion. Recovery of cells is therefore substantially lower than would be possible if the organs were thoroughly digested. It is found that increased perfusion flow rate, occlusion of at least one major blood vessel leading out of the organ, increased enzymatic digestion time, and vigorous tissue dissociation techniques can be combined to afford a uniquely high yield of viable cells.