Mice transgenic for a tetracycline-inducible transcriptional activator

US5912411A · kind A · utility

Assignee

• Universität Heidelberg · DE

Inventors

• Hermann Bujard · Heidelberg, DE
• Manfred Gossen · Corona, US

Key dates

• Filing date: Jun 7, 1995
• Grant date: Jun 15, 1999
• Expiry date: Jun 7, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2830/32
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Transgenic mice carrying a transgene comprising a nucleic acid molecule encoding protein useful for regulating the expression of genes in eukaryotic cells in a highly controlled manner are disclosed. In the regulatory system of the invention, transcription of a tet operator-linked nucleotide sequence is stimulated by a transcriptional activator fusion protein composed of two polypeptides, a first polypeptide which binds to tet operator sequences in the presence of tetracycline operatively linked to a second polypeptide activates transcription in eukaryotic cells. In a preferred embodiment, the transgene encoding the transcriptional activator fusion protein is integrated at a predetermined location within the chromosome of the transgenic mouse.