Materials and methods for the immobilization of bioactive species onto polymeric substrates
US5914182A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jun 3, 1996 |
| Grant date | Jun 22, 1999 |
| Priority date | — |
| Expiry date | Jun 3, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T428/31544
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed to support members having surfaces that are rendered hydrophilic for use as a substrate for the immobilization of bioactive species thereon. The hydrophilic surfaces are chemically stable on the support member. The surfaces are also chemically variable to provide a variety of chemically functional groups for immobilization of bioactive species thereto. The surfaces comprise polymeric surfactants attached onto the surfaces of a support member and covalently cross-linked thereon to form a first layer. Hydrophilic polymers are then attached to the first layer on the support member to form a second layer thereon. The second layer is used to enhance the hydrophilicity of a support member, as well as, to provide a substrate for immobilizing bioactive species thereto. Methods for forming the first and second layers are also provided. In addition methods for attaching bioactive substances to the hydrophilic polymers are provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.