Efficiency soybean transformation protocol
US5914451A · kind A · utility
112Cited by
6References
12Claims
0Family size
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Key dates
| Filing date | Apr 6, 1998 |
| Grant date | Jun 22, 1999 |
| Priority date | — |
| Expiry date | Apr 6, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8275
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A transformation protocol is described for the particle mediated transformation of soybean. This protocol is based on gene delivery into the growing meristem of a soybean embryo. Prior transformation protocols based on meristematic gene delivery in soybean did not depend on selection due to difficulties in using selection agents in differentiated tissue in soybean. It has been found that a post bombardment culture with glyphosate selection can dramatically increase the efficiency of such a transformation protocol.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.